Schedular

序号 领域 具体内容
1 Introduction Introduction
Medical revolutions in Biology
Biological applications in forensics, history, and agriculture
The diversity of life
An overvie of evolution and a comparison of prokaryotic and eukaryotic cells
The fundamental principles and intellectual framework of biology
2 Biochemistry Biochemistry of life
Framework, Buchner, and Fractionating life
Molecular composition of cells
Covalent bonds
Non-covalent bonds
Lipids and phospholipids: creating boundaries
High energy molecules: ATP and carbohydrates
3   Proteins and Protein Structure
Amazing proteins: primary structure
Meet the amino acids
Secondary structure
Tertiary and quaternary structure
4   Enzymes
Design a channel protein
Enzymes and biochemical reactions
What do enzymes do?
How do enzymes work?
Influenza virus F tricks of a burglar
5   Pathways: Glycolysis
The energetics of pathways
Logical tricks of pathways
Glycolysis: a pathway to break down sugar
Regulation of pathways
Cellular respiration and fermentation
6 Genetics Mendel（孟德尔）
Background: Who was Mendel? Why Peas?（背景知识：谁是孟德尔？为什么是豌豆？）
Mendel’s Experiments: controls and crosses（孟德尔的实验：对照组合杂交）
Definitions（定义）
Multiple traits: Mendel’s second law（多形状：孟德尔的第二法则）
Cytology（细胞学）
The chromosomal theory of inheritance（染色体遗传理论）
7   Rediscovery of Mendel and advances by TH Morgan
Meiosis（减数分裂）
Fruit flies and linkage（果蝇和连锁）
Linkage Maps（连锁图谱）
Linkage Mapping（连锁作图）
Sex chromosomes and sex linkage（性染色体和伴性遗传）
8   Basics of human genetics
X-linked recessive inheritance（X-相关隐性遗传）
Autosomal dominant inheritance（常染色体显性遗传）
Autosomal recessive inheritance（常染色体隐性遗传）
Real human genetics
Garrod and inborn errors of metabolism
9   Biochemical Genetics
Garrod, Beadle, Tatum and the link between genetics and biochemistry
Yeast as a model organism
How to use genetics to study biochemistry: A mutant hunt
Tricks of a mutant hunt
Characterizing mutants: test of dominance
Characterizing mutants: complementation test
Characterizing mutants: epistasis test
10 Molecular Biology DNA as the hereditary material
The Transforming principle
Structure of DNA: nucleotides and base-pairing
Bacterial viruses
DNA structure, the race
11   DNA Replication
Meselson and Stahl
Details of DNA replication
Additional details of DNA replication: topography and other enzymes
Additional details of DNA replication: fidelity
Kornberg’s enzyme
12   Central Dogma: Transcription and Translation
RNA
Transcription: making RNA copy of DNA
Translation: making a polypeptide from RNA
Peering back in time.
13   Variations on the Central Dogma
Replication in different organisms
Transcription in different organisms
Translation in different organisms
14   A tale of two genes: β-galactosidase and β-globin
β-galactosidase in E. coli
Lac operon, lactose regulation
Lac operon, glucose regulation
Hormone receptors in mammals
β-globin gene structure.
β-globin mutations
The β-globin region
15 Recombinant DNA Cloning: Purifying a gene
Overview
Cutting and pasting molecules of DNA
Vectors
Transformation of host cells
Selection and creating a library
16   Finding a specific gene in the library
Review/Overview
Tricks for cloning
Different cloning vectors and source DNA
Finding your gene by complementation
Finding your gene by protein expression
17   Analyzing a gene
Gel electrophoresis
DNA sequencing, the concept
DNA sequencing, implementation
Polymerase Chain Reaction (PCR)
18 Genomics Human genome and positional cloning
Recombinant DNA review
Finding your gene: human Mendelian diseases
Finding markers across the genome for positional cloning
The Human Genome Project, genome assembly and analysis
Improvements since the Human Genome project
Improvements in DNA sequencing
19   Secrets of the human genome
Tour of the genome: the genomic landscape
Evolutionary comparison
Evolutionary comparison as a tool for biomedical research
DNA polymorphisms within humans
20   Observing
DNA polymorphism in medicine: Mendelian disease
DNA polymorphisms: polygenic disease 3
RNA variation
Protein localization on the genome
21 Completing the Triangle Perturbing the genome to probe function
Adding and subtracting genes
RNA interference
Modern genome editing: TALEN proteins and CRSPR
22 Rational Medicine Familial hypercholesterolemia
Heart disease
Cholesterol
Lipoprotein particles
Connections to heart disease
Genetics of cholesterol levels
Rational therapy for FH heterozygotes
Modern strategies: PCSK9 and HDL
23   Cancer
Cancer
Regulation of cell growth: growth factors and receptors
Regulation of cell growth: Ras
Regulation of cell growth: Ras signaling
Mutations that cause cancer
Anti-cancer therapy
24   Science and Society
DNA and law
Other forensic technologies
Gene patenting

Notes

Lecture 7: Rediscovery of Mendel and advances by TH Morgan

  • Mendel’s 2nd Law(Completely Independent) vs Chrom Theory(Completely Dependent)
  • Recombination
  • Mitosis(有丝分裂): G1 –> S/G2 –> Mitosis –> G1
  • Meiosis(减数分裂): G1 –> S/G2 (recombination could occur) –> Meiosis I –> Meiosis II –> End